Determination of aromatic amines through the use of tandem mass spectrometry coupled to gas phase chromatography

Determination of aromatic amines through the use of tandem mass spectrometry coupled to gas phase chromatography

The number of compounds of regulatory interest in cigarette smoke has increased in recent years. The US FDA Tobacco Products Scientific Advisory Committee (TPSAC) proposed list includes six aromatic amines compounds. The established GC-MS methods for the analysis of aromatic amines involve complex sample preparation and encompass only a portion of the six compounds of interest. The objective of this study was to develop a single analytical method capable of determining all HPHC aromatic amines (o Anisidine, o Toluidine, 2,6 Dimethylaniline, 1 Aminonaphthalene, 2 Aminonaphthalene, and 4 Aminobiphenyl). Gas chromatography coupled with tandem mass spectrometry was used to measure these compounds. Cambridge pads were extracted with aqueous acid and the extracts were subsequently processed through two solid phase extraction steps and derivatized prior to analysis. The aromatic amines were separated on an Agilent 7890 using an HP-5MS dual-column system with post-run backflushing through the first column. Detection followed on an Agilent 7000 QQQ tandem mass spectrometer. The mass spectrometer was operated in electron impact mode and all compounds were detected using multiple reaction monitoring (MRM). In general, all compounds gave levels of detection less than 1 ng/mL. Calibration and other analytical details will be presented for all compounds.

Authors and Affiliations:
BROWN S.S.; GILLMAN I.G.
Enthalpy Analytical, Durham, NC, USA

Citation:
TSRC, Tob. Sci. Res. Conf., 2012, 66, abstr. 12